Localization of Thioredoxin-Interacting Protein in Aging and Alzheimer’s Disease Brains

Thioredoxin-Interacting Protein (TXNIP) has been shown to have significant pathogenic roles in many human diseases, particularly those associated with diabetes and hyperglycemia. Its main mode of action is sequester thioredoxins, resulting enhanced oxidative stress. The aim this study was identify if cellular expression TXNIP aged Alzheimer’s disease (AD) brains correlated pathological structures. This employed fixed tissue sections protein extracts temporal cortex from AD control brains. Studies light fluorescent immunohistochemical techniques using the monoclonal antibody JY2 Immunoblots were used quantify relative amounts brain extracts. major finding identification immunoreactivity selective neuronal populations structures, non-AD In brains, less but increased numbers TXNIP-positive plaque-associated microglia observed. Immunoblot analyses showed no increase levels samples tested. conclusion, identified altered patterns progression pathology.